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The performance of the ARK assay was evaluated on the Beckman AU Automated Clinical Chemistry Analyzer. Precision, limit of quantitation, recovery, . were collected and sera were separated and stored at − C. All the samples were measured collectively by auto-analyzer AU (Beckman-Coulter). Beckman Coulter, AU Clinical System (Chemistry Low Volume ) Analytes . Free Download Buku Metode Penelitian Sugiyono Ebook.
No restrictions were placed on age or reproductive status. The second group comprised Miniature schnauzers recruited to the University of Minnesota UMN Veterinary Medical Center from to for both this study and concurrent genetic studies in the breed. Dogs were excluded if they had a diagnosis of hyperadrenocorticism, hypothyroidism or diabetes mellitus, or were receiving corticosteroids.
Owner written informed consent was obtained for each study participant. Triglyceride and biochemical measurements Dogs were fasted for 12—18 h prior to sample collection. Blood was collected in tubes without additives. The UMN group had blood collected into a syringe with dry lithium heparin for determination of creatinine, glucose and electrolytes using a blood gas analyzer i-STAT 1, Abbott Point of Care.
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Serum total thyroxine, total triiodothyronine, free thyroxine, thyroid stimulating hormone, and triiodothyronine and thyroxine autoantibody concentrations, were also measured in the OSU dogs Michigan State University Animal Health Diagnostic Laboratory. The owners of the UMN dogs with HTG were asked to return for a second study visit for determination of serum total thyroxine concentration AU Chemistry analyzer, Beckman Coulter and a urine cortisol-to-creatinine ratio Marshfield Labs Veterinary Services , but these tests were not required for study participation.
Urinary protein determination Urine was collected by cystocentesis or mid-stream free-catch into a sterile container. Samples were stored at room temperature and a urinalysis was performed within 4 h.
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Blood was obtained via jugular venipuncture prior to and 10 min after heparin administration and placed into tubes containing ethylene diamine tetra-acetic acid EDTA on ice. The decrease in total lipase activity after addition of 5D2 indicates LPL activity, while the remaining activity reflects HL.
A bovine milk lipase and a human post-heparin standard were included with each assay. Statistical analysis Data distribution was inspected with Q-Q plots and assessed for normality with the Shapiro—Wilk test.
Body condition score BCS, 1—5 scale is also reported as median range. Pearson correlation coefficients r were calculated to assess relationships between variables of interest.
Associations of age years , sex male versus female and BCS were analyzed in a multivariable regression with logTG as the outcome. LogUPC was analyzed as a parallel outcome.
A simple regression was used to test the relationship between serum cholesterol and UPC; cholesterol was not included in the multivariable regressions due to missing values. Significance was assessed using Type II tests and the coefficient of determination R2 was calculated to assess model fit.
Analyses were performed using R software for statistical computing.
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Both sizes have a USB port automatic or manual storage of log files, to speed up your process.The second group comprised Miniature schnauzers recruited to the University of Minnesota UMN Veterinary Medical Center from to for both this study and concurrent genetic studies in the breed.
LogUPC was analyzed as a parallel outcome. Listening to customers to optimize the product User-friendliness: every major lab equipment supplier knows it is the number one priority in product development.
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Serum total thyroxine, total triiodothyronine, free thyroxine, thyroid stimulating hormone, and triiodothyronine and thyroxine autoantibody concentrations, were also measured in the OSU dogs Michigan State University Animal Health Diagnostic Laboratory.